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GENETYX CORPORATION genetyx-mac 21.1.0 software
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Related Articles

Expressing:

Article Title: Reorganization of the ancestral sex-determining regions during the evolution of trioecy in Pleodorina starrii
Article Snippet: The MTD1 sequences of Chlamydomonas reinhardtii (CrMTD1), Gonium pectorale (GpMTD1), Yamagishiella unicocca (YuMTD1), Eudorina sp. (EuMTD1), Volvox reticuliferus (VrMTD1), Volvox africanus (VaMTD1) and Pleodorina starrii (PsMTD1, identified in this study) were aligned using the MUSCLE program built in the GENETYX-MAC software ver.

Article Title: HSF1 is required for cellular adaptation to daily temperature fluctuations
Article Snippet: The predicted amino acid sequences of HSFs from various vertebrate species were compared using GENETYX-MAC software (GENETYX Co., Tokyo, Japan).

Article Title: The CGA codon decoding through tRNA Arg (ICG) supply governed by Tad2/Tad3 in Saccharomyces cerevisiae.
Article Snippet: The detection by the anti-PGK1 antibody was used as a control. (B) Alignment of tR(ACG) gene sequences of S. cerevisiae from Saccharomyces genome database (SGD project, reference genome sequence R64-2-1) (tR(ACG)D, tR(ACG)E, tR(ACG)K, tR(ACG)L, tR(ACG)O and tR (ACG)J) aligned by GENETYX-MAC software (GENETYX Corporation, Tokyo, Japan).

Article Title: Real-time PCR assays to detect 10 Shiga toxin subtype (Stx1a, Stx1c, Stx1d, Stx2a, Stx2b, Stx2c, Stx2d, Stx2e, Stx2f, and Stx2g) genes.
Article Snippet: Todevelop subtypingmethods for Shiga toxin (Stx)1a, Stx1c, Stx1d, Stx2a, Stx2b, Stx2c, Stx2d, Stx2e, Stx2f, and Stx2g genes for epidemiological analyses of Shiga toxin-producing Escherichia coli (STEC), we developed 10 simplex realtimepolymerase chain reaction (PCR) assayswith reference to 284 valid stx sequences and evaluated their specificity and quantitative accuracy using STEC and non-STEC isolates and recombinant plasmids, respectively.. Three stx1 and 5 stx2 subtype genes, except for stx2c and stx2d, were detected with high specificity using STEC isolates.. However, some stx2a sequences potentially being close to both Stx2a and Stx2d cluster in neighbor-joining cluster analysis were positive for stx2a and stx2d by real-timePCR.

Article Title: Common host factors for internal ribosomal entry site-mediated translation of viral genomic RNA: An investigation in foot-and-mouth disease and classical swine fever viruses.
Article Snippet: DNA sequence characterization was performed using GENETYX-Mac software (GENETYX Co., Tokyo, Japan) and GENBANK.

Article Title: Use of universal primers for the 18S ribosomal RNA gene and whole soil DNAs to reveal the taxonomic structures of soil nematodes by high-throughput amplicon sequencing
Article Snippet: In brief, the nucleotide sequences were aligned, and phylogenetic trees were constructed using the BOOTSTRAP N-J TREE algorithm (bootstrap: 1000 replicates) with the ClustalX (version 2.1) package ( http://www.clustal.org/clustal2/ ) in the Genetyx-MAC software (version 19, Genetyx Co.) [ ].

Article Title: Evaluation of VP4-VP2 sequencing for molecular typing of human enteroviruses.
Article Snippet: Furthermore, pairwise identity comparison with gap was performed using the GENETYX-Mac software (version 20; GENETYX, Tokyo, Japan).

Article Title: Structural analyses of the GI.4 norovirus by cryo-electron microscopy and X-ray crystallography revealing binding sites for human monoclonal antibodies.
Article Snippet: Amino acid sequences of the P1 and P2 subdomains were aligned with the help of GENETYX-MAC software (Genetyx Corp., Tokyo).

Binding Assay:

Article Title: Reorganization of the ancestral sex-determining regions during the evolution of trioecy in Pleodorina starrii
Article Snippet: The MTD1 sequences of Chlamydomonas reinhardtii (CrMTD1), Gonium pectorale (GpMTD1), Yamagishiella unicocca (YuMTD1), Eudorina sp. (EuMTD1), Volvox reticuliferus (VrMTD1), Volvox africanus (VaMTD1) and Pleodorina starrii (PsMTD1, identified in this study) were aligned using the MUSCLE program built in the GENETYX-MAC software ver.

Article Title: HSF1 is required for cellular adaptation to daily temperature fluctuations
Article Snippet: The predicted amino acid sequences of HSFs from various vertebrate species were compared using GENETYX-MAC software (GENETYX Co., Tokyo, Japan).

Article Title: The CGA codon decoding through tRNA Arg (ICG) supply governed by Tad2/Tad3 in Saccharomyces cerevisiae.
Article Snippet: The detection by the anti-PGK1 antibody was used as a control. (B) Alignment of tR(ACG) gene sequences of S. cerevisiae from Saccharomyces genome database (SGD project, reference genome sequence R64-2-1) (tR(ACG)D, tR(ACG)E, tR(ACG)K, tR(ACG)L, tR(ACG)O and tR (ACG)J) aligned by GENETYX-MAC software (GENETYX Corporation, Tokyo, Japan).

Article Title: Real-time PCR assays to detect 10 Shiga toxin subtype (Stx1a, Stx1c, Stx1d, Stx2a, Stx2b, Stx2c, Stx2d, Stx2e, Stx2f, and Stx2g) genes.
Article Snippet: Todevelop subtypingmethods for Shiga toxin (Stx)1a, Stx1c, Stx1d, Stx2a, Stx2b, Stx2c, Stx2d, Stx2e, Stx2f, and Stx2g genes for epidemiological analyses of Shiga toxin-producing Escherichia coli (STEC), we developed 10 simplex realtimepolymerase chain reaction (PCR) assayswith reference to 284 valid stx sequences and evaluated their specificity and quantitative accuracy using STEC and non-STEC isolates and recombinant plasmids, respectively.. Three stx1 and 5 stx2 subtype genes, except for stx2c and stx2d, were detected with high specificity using STEC isolates.. However, some stx2a sequences potentially being close to both Stx2a and Stx2d cluster in neighbor-joining cluster analysis were positive for stx2a and stx2d by real-timePCR.

Article Title: Common host factors for internal ribosomal entry site-mediated translation of viral genomic RNA: An investigation in foot-and-mouth disease and classical swine fever viruses.
Article Snippet: DNA sequence characterization was performed using GENETYX-Mac software (GENETYX Co., Tokyo, Japan) and GENBANK.

Article Title: Use of universal primers for the 18S ribosomal RNA gene and whole soil DNAs to reveal the taxonomic structures of soil nematodes by high-throughput amplicon sequencing
Article Snippet: In brief, the nucleotide sequences were aligned, and phylogenetic trees were constructed using the BOOTSTRAP N-J TREE algorithm (bootstrap: 1000 replicates) with the ClustalX (version 2.1) package ( http://www.clustal.org/clustal2/ ) in the Genetyx-MAC software (version 19, Genetyx Co.) [ ].

Article Title: Evaluation of VP4-VP2 sequencing for molecular typing of human enteroviruses.
Article Snippet: Furthermore, pairwise identity comparison with gap was performed using the GENETYX-Mac software (version 20; GENETYX, Tokyo, Japan).

Article Title: Structural analyses of the GI.4 norovirus by cryo-electron microscopy and X-ray crystallography revealing binding sites for human monoclonal antibodies.
Article Snippet: Amino acid sequences of the P1 and P2 subdomains were aligned with the help of GENETYX-MAC software (Genetyx Corp., Tokyo).

Activation Assay:

Article Title: Reorganization of the ancestral sex-determining regions during the evolution of trioecy in Pleodorina starrii
Article Snippet: The MTD1 sequences of Chlamydomonas reinhardtii (CrMTD1), Gonium pectorale (GpMTD1), Yamagishiella unicocca (YuMTD1), Eudorina sp. (EuMTD1), Volvox reticuliferus (VrMTD1), Volvox africanus (VaMTD1) and Pleodorina starrii (PsMTD1, identified in this study) were aligned using the MUSCLE program built in the GENETYX-MAC software ver.

Article Title: HSF1 is required for cellular adaptation to daily temperature fluctuations
Article Snippet: The predicted amino acid sequences of HSFs from various vertebrate species were compared using GENETYX-MAC software (GENETYX Co., Tokyo, Japan).

Article Title: The CGA codon decoding through tRNA Arg (ICG) supply governed by Tad2/Tad3 in Saccharomyces cerevisiae.
Article Snippet: The detection by the anti-PGK1 antibody was used as a control. (B) Alignment of tR(ACG) gene sequences of S. cerevisiae from Saccharomyces genome database (SGD project, reference genome sequence R64-2-1) (tR(ACG)D, tR(ACG)E, tR(ACG)K, tR(ACG)L, tR(ACG)O and tR (ACG)J) aligned by GENETYX-MAC software (GENETYX Corporation, Tokyo, Japan).

Article Title: Real-time PCR assays to detect 10 Shiga toxin subtype (Stx1a, Stx1c, Stx1d, Stx2a, Stx2b, Stx2c, Stx2d, Stx2e, Stx2f, and Stx2g) genes.
Article Snippet: Todevelop subtypingmethods for Shiga toxin (Stx)1a, Stx1c, Stx1d, Stx2a, Stx2b, Stx2c, Stx2d, Stx2e, Stx2f, and Stx2g genes for epidemiological analyses of Shiga toxin-producing Escherichia coli (STEC), we developed 10 simplex realtimepolymerase chain reaction (PCR) assayswith reference to 284 valid stx sequences and evaluated their specificity and quantitative accuracy using STEC and non-STEC isolates and recombinant plasmids, respectively.. Three stx1 and 5 stx2 subtype genes, except for stx2c and stx2d, were detected with high specificity using STEC isolates.. However, some stx2a sequences potentially being close to both Stx2a and Stx2d cluster in neighbor-joining cluster analysis were positive for stx2a and stx2d by real-timePCR.

Article Title: Common host factors for internal ribosomal entry site-mediated translation of viral genomic RNA: An investigation in foot-and-mouth disease and classical swine fever viruses.
Article Snippet: DNA sequence characterization was performed using GENETYX-Mac software (GENETYX Co., Tokyo, Japan) and GENBANK.

Article Title: Use of universal primers for the 18S ribosomal RNA gene and whole soil DNAs to reveal the taxonomic structures of soil nematodes by high-throughput amplicon sequencing
Article Snippet: In brief, the nucleotide sequences were aligned, and phylogenetic trees were constructed using the BOOTSTRAP N-J TREE algorithm (bootstrap: 1000 replicates) with the ClustalX (version 2.1) package ( http://www.clustal.org/clustal2/ ) in the Genetyx-MAC software (version 19, Genetyx Co.) [ ].

Article Title: Evaluation of VP4-VP2 sequencing for molecular typing of human enteroviruses.
Article Snippet: Furthermore, pairwise identity comparison with gap was performed using the GENETYX-Mac software (version 20; GENETYX, Tokyo, Japan).

Article Title: Structural analyses of the GI.4 norovirus by cryo-electron microscopy and X-ray crystallography revealing binding sites for human monoclonal antibodies.
Article Snippet: Amino acid sequences of the P1 and P2 subdomains were aligned with the help of GENETYX-MAC software (Genetyx Corp., Tokyo).

Luciferase:

Article Title: Reorganization of the ancestral sex-determining regions during the evolution of trioecy in Pleodorina starrii
Article Snippet: The MTD1 sequences of Chlamydomonas reinhardtii (CrMTD1), Gonium pectorale (GpMTD1), Yamagishiella unicocca (YuMTD1), Eudorina sp. (EuMTD1), Volvox reticuliferus (VrMTD1), Volvox africanus (VaMTD1) and Pleodorina starrii (PsMTD1, identified in this study) were aligned using the MUSCLE program built in the GENETYX-MAC software ver.

Article Title: HSF1 is required for cellular adaptation to daily temperature fluctuations
Article Snippet: The predicted amino acid sequences of HSFs from various vertebrate species were compared using GENETYX-MAC software (GENETYX Co., Tokyo, Japan).

Article Title: The CGA codon decoding through tRNA Arg (ICG) supply governed by Tad2/Tad3 in Saccharomyces cerevisiae.
Article Snippet: The detection by the anti-PGK1 antibody was used as a control. (B) Alignment of tR(ACG) gene sequences of S. cerevisiae from Saccharomyces genome database (SGD project, reference genome sequence R64-2-1) (tR(ACG)D, tR(ACG)E, tR(ACG)K, tR(ACG)L, tR(ACG)O and tR (ACG)J) aligned by GENETYX-MAC software (GENETYX Corporation, Tokyo, Japan).

Article Title: Real-time PCR assays to detect 10 Shiga toxin subtype (Stx1a, Stx1c, Stx1d, Stx2a, Stx2b, Stx2c, Stx2d, Stx2e, Stx2f, and Stx2g) genes.
Article Snippet: Todevelop subtypingmethods for Shiga toxin (Stx)1a, Stx1c, Stx1d, Stx2a, Stx2b, Stx2c, Stx2d, Stx2e, Stx2f, and Stx2g genes for epidemiological analyses of Shiga toxin-producing Escherichia coli (STEC), we developed 10 simplex realtimepolymerase chain reaction (PCR) assayswith reference to 284 valid stx sequences and evaluated their specificity and quantitative accuracy using STEC and non-STEC isolates and recombinant plasmids, respectively.. Three stx1 and 5 stx2 subtype genes, except for stx2c and stx2d, were detected with high specificity using STEC isolates.. However, some stx2a sequences potentially being close to both Stx2a and Stx2d cluster in neighbor-joining cluster analysis were positive for stx2a and stx2d by real-timePCR.

Article Title: Common host factors for internal ribosomal entry site-mediated translation of viral genomic RNA: An investigation in foot-and-mouth disease and classical swine fever viruses.
Article Snippet: DNA sequence characterization was performed using GENETYX-Mac software (GENETYX Co., Tokyo, Japan) and GENBANK.

Article Title: Use of universal primers for the 18S ribosomal RNA gene and whole soil DNAs to reveal the taxonomic structures of soil nematodes by high-throughput amplicon sequencing
Article Snippet: In brief, the nucleotide sequences were aligned, and phylogenetic trees were constructed using the BOOTSTRAP N-J TREE algorithm (bootstrap: 1000 replicates) with the ClustalX (version 2.1) package ( http://www.clustal.org/clustal2/ ) in the Genetyx-MAC software (version 19, Genetyx Co.) [ ].

Article Title: Evaluation of VP4-VP2 sequencing for molecular typing of human enteroviruses.
Article Snippet: Furthermore, pairwise identity comparison with gap was performed using the GENETYX-Mac software (version 20; GENETYX, Tokyo, Japan).

Article Title: Structural analyses of the GI.4 norovirus by cryo-electron microscopy and X-ray crystallography revealing binding sites for human monoclonal antibodies.
Article Snippet: Amino acid sequences of the P1 and P2 subdomains were aligned with the help of GENETYX-MAC software (Genetyx Corp., Tokyo).

Generated:

Article Title: Reorganization of the ancestral sex-determining regions during the evolution of trioecy in Pleodorina starrii
Article Snippet: The MTD1 sequences of Chlamydomonas reinhardtii (CrMTD1), Gonium pectorale (GpMTD1), Yamagishiella unicocca (YuMTD1), Eudorina sp. (EuMTD1), Volvox reticuliferus (VrMTD1), Volvox africanus (VaMTD1) and Pleodorina starrii (PsMTD1, identified in this study) were aligned using the MUSCLE program built in the GENETYX-MAC software ver.

Article Title: HSF1 is required for cellular adaptation to daily temperature fluctuations
Article Snippet: The predicted amino acid sequences of HSFs from various vertebrate species were compared using GENETYX-MAC software (GENETYX Co., Tokyo, Japan).

Article Title: The CGA codon decoding through tRNA Arg (ICG) supply governed by Tad2/Tad3 in Saccharomyces cerevisiae.
Article Snippet: The detection by the anti-PGK1 antibody was used as a control. (B) Alignment of tR(ACG) gene sequences of S. cerevisiae from Saccharomyces genome database (SGD project, reference genome sequence R64-2-1) (tR(ACG)D, tR(ACG)E, tR(ACG)K, tR(ACG)L, tR(ACG)O and tR (ACG)J) aligned by GENETYX-MAC software (GENETYX Corporation, Tokyo, Japan).

Article Title: Real-time PCR assays to detect 10 Shiga toxin subtype (Stx1a, Stx1c, Stx1d, Stx2a, Stx2b, Stx2c, Stx2d, Stx2e, Stx2f, and Stx2g) genes.
Article Snippet: Todevelop subtypingmethods for Shiga toxin (Stx)1a, Stx1c, Stx1d, Stx2a, Stx2b, Stx2c, Stx2d, Stx2e, Stx2f, and Stx2g genes for epidemiological analyses of Shiga toxin-producing Escherichia coli (STEC), we developed 10 simplex realtimepolymerase chain reaction (PCR) assayswith reference to 284 valid stx sequences and evaluated their specificity and quantitative accuracy using STEC and non-STEC isolates and recombinant plasmids, respectively.. Three stx1 and 5 stx2 subtype genes, except for stx2c and stx2d, were detected with high specificity using STEC isolates.. However, some stx2a sequences potentially being close to both Stx2a and Stx2d cluster in neighbor-joining cluster analysis were positive for stx2a and stx2d by real-timePCR.

Article Title: Common host factors for internal ribosomal entry site-mediated translation of viral genomic RNA: An investigation in foot-and-mouth disease and classical swine fever viruses.
Article Snippet: DNA sequence characterization was performed using GENETYX-Mac software (GENETYX Co., Tokyo, Japan) and GENBANK.

Article Title: Use of universal primers for the 18S ribosomal RNA gene and whole soil DNAs to reveal the taxonomic structures of soil nematodes by high-throughput amplicon sequencing
Article Snippet: In brief, the nucleotide sequences were aligned, and phylogenetic trees were constructed using the BOOTSTRAP N-J TREE algorithm (bootstrap: 1000 replicates) with the ClustalX (version 2.1) package ( http://www.clustal.org/clustal2/ ) in the Genetyx-MAC software (version 19, Genetyx Co.) [ ].

Article Title: Evaluation of VP4-VP2 sequencing for molecular typing of human enteroviruses.
Article Snippet: Furthermore, pairwise identity comparison with gap was performed using the GENETYX-Mac software (version 20; GENETYX, Tokyo, Japan).

Article Title: Structural analyses of the GI.4 norovirus by cryo-electron microscopy and X-ray crystallography revealing binding sites for human monoclonal antibodies.
Article Snippet: Amino acid sequences of the P1 and P2 subdomains were aligned with the help of GENETYX-MAC software (Genetyx Corp., Tokyo).

Infection:

Article Title: Reorganization of the ancestral sex-determining regions during the evolution of trioecy in Pleodorina starrii
Article Snippet: The MTD1 sequences of Chlamydomonas reinhardtii (CrMTD1), Gonium pectorale (GpMTD1), Yamagishiella unicocca (YuMTD1), Eudorina sp. (EuMTD1), Volvox reticuliferus (VrMTD1), Volvox africanus (VaMTD1) and Pleodorina starrii (PsMTD1, identified in this study) were aligned using the MUSCLE program built in the GENETYX-MAC software ver.

Article Title: HSF1 is required for cellular adaptation to daily temperature fluctuations
Article Snippet: The predicted amino acid sequences of HSFs from various vertebrate species were compared using GENETYX-MAC software (GENETYX Co., Tokyo, Japan).

Article Title: The CGA codon decoding through tRNA Arg (ICG) supply governed by Tad2/Tad3 in Saccharomyces cerevisiae.
Article Snippet: The detection by the anti-PGK1 antibody was used as a control. (B) Alignment of tR(ACG) gene sequences of S. cerevisiae from Saccharomyces genome database (SGD project, reference genome sequence R64-2-1) (tR(ACG)D, tR(ACG)E, tR(ACG)K, tR(ACG)L, tR(ACG)O and tR (ACG)J) aligned by GENETYX-MAC software (GENETYX Corporation, Tokyo, Japan).

Article Title: Real-time PCR assays to detect 10 Shiga toxin subtype (Stx1a, Stx1c, Stx1d, Stx2a, Stx2b, Stx2c, Stx2d, Stx2e, Stx2f, and Stx2g) genes.
Article Snippet: Todevelop subtypingmethods for Shiga toxin (Stx)1a, Stx1c, Stx1d, Stx2a, Stx2b, Stx2c, Stx2d, Stx2e, Stx2f, and Stx2g genes for epidemiological analyses of Shiga toxin-producing Escherichia coli (STEC), we developed 10 simplex realtimepolymerase chain reaction (PCR) assayswith reference to 284 valid stx sequences and evaluated their specificity and quantitative accuracy using STEC and non-STEC isolates and recombinant plasmids, respectively.. Three stx1 and 5 stx2 subtype genes, except for stx2c and stx2d, were detected with high specificity using STEC isolates.. However, some stx2a sequences potentially being close to both Stx2a and Stx2d cluster in neighbor-joining cluster analysis were positive for stx2a and stx2d by real-timePCR.

Article Title: Common host factors for internal ribosomal entry site-mediated translation of viral genomic RNA: An investigation in foot-and-mouth disease and classical swine fever viruses.
Article Snippet: DNA sequence characterization was performed using GENETYX-Mac software (GENETYX Co., Tokyo, Japan) and GENBANK.

Article Title: Use of universal primers for the 18S ribosomal RNA gene and whole soil DNAs to reveal the taxonomic structures of soil nematodes by high-throughput amplicon sequencing
Article Snippet: In brief, the nucleotide sequences were aligned, and phylogenetic trees were constructed using the BOOTSTRAP N-J TREE algorithm (bootstrap: 1000 replicates) with the ClustalX (version 2.1) package ( http://www.clustal.org/clustal2/ ) in the Genetyx-MAC software (version 19, Genetyx Co.) [ ].

Article Title: Evaluation of VP4-VP2 sequencing for molecular typing of human enteroviruses.
Article Snippet: Furthermore, pairwise identity comparison with gap was performed using the GENETYX-Mac software (version 20; GENETYX, Tokyo, Japan).

Article Title: Structural analyses of the GI.4 norovirus by cryo-electron microscopy and X-ray crystallography revealing binding sites for human monoclonal antibodies.
Article Snippet: Amino acid sequences of the P1 and P2 subdomains were aligned with the help of GENETYX-MAC software (Genetyx Corp., Tokyo).

Control:

Article Title: Reorganization of the ancestral sex-determining regions during the evolution of trioecy in Pleodorina starrii
Article Snippet: The MTD1 sequences of Chlamydomonas reinhardtii (CrMTD1), Gonium pectorale (GpMTD1), Yamagishiella unicocca (YuMTD1), Eudorina sp. (EuMTD1), Volvox reticuliferus (VrMTD1), Volvox africanus (VaMTD1) and Pleodorina starrii (PsMTD1, identified in this study) were aligned using the MUSCLE program built in the GENETYX-MAC software ver.

Article Title: HSF1 is required for cellular adaptation to daily temperature fluctuations
Article Snippet: The predicted amino acid sequences of HSFs from various vertebrate species were compared using GENETYX-MAC software (GENETYX Co., Tokyo, Japan).

Article Title: The CGA codon decoding through tRNA Arg (ICG) supply governed by Tad2/Tad3 in Saccharomyces cerevisiae.
Article Snippet: The detection by the anti-PGK1 antibody was used as a control. (B) Alignment of tR(ACG) gene sequences of S. cerevisiae from Saccharomyces genome database (SGD project, reference genome sequence R64-2-1) (tR(ACG)D, tR(ACG)E, tR(ACG)K, tR(ACG)L, tR(ACG)O and tR (ACG)J) aligned by GENETYX-MAC software (GENETYX Corporation, Tokyo, Japan).

Article Title: Real-time PCR assays to detect 10 Shiga toxin subtype (Stx1a, Stx1c, Stx1d, Stx2a, Stx2b, Stx2c, Stx2d, Stx2e, Stx2f, and Stx2g) genes.
Article Snippet: Todevelop subtypingmethods for Shiga toxin (Stx)1a, Stx1c, Stx1d, Stx2a, Stx2b, Stx2c, Stx2d, Stx2e, Stx2f, and Stx2g genes for epidemiological analyses of Shiga toxin-producing Escherichia coli (STEC), we developed 10 simplex realtimepolymerase chain reaction (PCR) assayswith reference to 284 valid stx sequences and evaluated their specificity and quantitative accuracy using STEC and non-STEC isolates and recombinant plasmids, respectively.. Three stx1 and 5 stx2 subtype genes, except for stx2c and stx2d, were detected with high specificity using STEC isolates.. However, some stx2a sequences potentially being close to both Stx2a and Stx2d cluster in neighbor-joining cluster analysis were positive for stx2a and stx2d by real-timePCR.

Article Title: Common host factors for internal ribosomal entry site-mediated translation of viral genomic RNA: An investigation in foot-and-mouth disease and classical swine fever viruses.
Article Snippet: DNA sequence characterization was performed using GENETYX-Mac software (GENETYX Co., Tokyo, Japan) and GENBANK.

Article Title: Use of universal primers for the 18S ribosomal RNA gene and whole soil DNAs to reveal the taxonomic structures of soil nematodes by high-throughput amplicon sequencing
Article Snippet: In brief, the nucleotide sequences were aligned, and phylogenetic trees were constructed using the BOOTSTRAP N-J TREE algorithm (bootstrap: 1000 replicates) with the ClustalX (version 2.1) package ( http://www.clustal.org/clustal2/ ) in the Genetyx-MAC software (version 19, Genetyx Co.) [ ].

Article Title: Evaluation of VP4-VP2 sequencing for molecular typing of human enteroviruses.
Article Snippet: Furthermore, pairwise identity comparison with gap was performed using the GENETYX-Mac software (version 20; GENETYX, Tokyo, Japan).

Article Title: Structural analyses of the GI.4 norovirus by cryo-electron microscopy and X-ray crystallography revealing binding sites for human monoclonal antibodies.
Article Snippet: Amino acid sequences of the P1 and P2 subdomains were aligned with the help of GENETYX-MAC software (Genetyx Corp., Tokyo).

Western Blot:

Article Title: Reorganization of the ancestral sex-determining regions during the evolution of trioecy in Pleodorina starrii
Article Snippet: The MTD1 sequences of Chlamydomonas reinhardtii (CrMTD1), Gonium pectorale (GpMTD1), Yamagishiella unicocca (YuMTD1), Eudorina sp. (EuMTD1), Volvox reticuliferus (VrMTD1), Volvox africanus (VaMTD1) and Pleodorina starrii (PsMTD1, identified in this study) were aligned using the MUSCLE program built in the GENETYX-MAC software ver.

Article Title: HSF1 is required for cellular adaptation to daily temperature fluctuations
Article Snippet: The predicted amino acid sequences of HSFs from various vertebrate species were compared using GENETYX-MAC software (GENETYX Co., Tokyo, Japan).

Article Title: The CGA codon decoding through tRNA Arg (ICG) supply governed by Tad2/Tad3 in Saccharomyces cerevisiae.
Article Snippet: The detection by the anti-PGK1 antibody was used as a control. (B) Alignment of tR(ACG) gene sequences of S. cerevisiae from Saccharomyces genome database (SGD project, reference genome sequence R64-2-1) (tR(ACG)D, tR(ACG)E, tR(ACG)K, tR(ACG)L, tR(ACG)O and tR (ACG)J) aligned by GENETYX-MAC software (GENETYX Corporation, Tokyo, Japan).

Article Title: Real-time PCR assays to detect 10 Shiga toxin subtype (Stx1a, Stx1c, Stx1d, Stx2a, Stx2b, Stx2c, Stx2d, Stx2e, Stx2f, and Stx2g) genes.
Article Snippet: Todevelop subtypingmethods for Shiga toxin (Stx)1a, Stx1c, Stx1d, Stx2a, Stx2b, Stx2c, Stx2d, Stx2e, Stx2f, and Stx2g genes for epidemiological analyses of Shiga toxin-producing Escherichia coli (STEC), we developed 10 simplex realtimepolymerase chain reaction (PCR) assayswith reference to 284 valid stx sequences and evaluated their specificity and quantitative accuracy using STEC and non-STEC isolates and recombinant plasmids, respectively.. Three stx1 and 5 stx2 subtype genes, except for stx2c and stx2d, were detected with high specificity using STEC isolates.. However, some stx2a sequences potentially being close to both Stx2a and Stx2d cluster in neighbor-joining cluster analysis were positive for stx2a and stx2d by real-timePCR.

Article Title: Common host factors for internal ribosomal entry site-mediated translation of viral genomic RNA: An investigation in foot-and-mouth disease and classical swine fever viruses.
Article Snippet: DNA sequence characterization was performed using GENETYX-Mac software (GENETYX Co., Tokyo, Japan) and GENBANK.

Article Title: Use of universal primers for the 18S ribosomal RNA gene and whole soil DNAs to reveal the taxonomic structures of soil nematodes by high-throughput amplicon sequencing
Article Snippet: In brief, the nucleotide sequences were aligned, and phylogenetic trees were constructed using the BOOTSTRAP N-J TREE algorithm (bootstrap: 1000 replicates) with the ClustalX (version 2.1) package ( http://www.clustal.org/clustal2/ ) in the Genetyx-MAC software (version 19, Genetyx Co.) [ ].

Article Title: Evaluation of VP4-VP2 sequencing for molecular typing of human enteroviruses.
Article Snippet: Furthermore, pairwise identity comparison with gap was performed using the GENETYX-Mac software (version 20; GENETYX, Tokyo, Japan).

Article Title: Structural analyses of the GI.4 norovirus by cryo-electron microscopy and X-ray crystallography revealing binding sites for human monoclonal antibodies.
Article Snippet: Amino acid sequences of the P1 and P2 subdomains were aligned with the help of GENETYX-MAC software (Genetyx Corp., Tokyo).

Stable Transfection:

Article Title: Reorganization of the ancestral sex-determining regions during the evolution of trioecy in Pleodorina starrii
Article Snippet: The MTD1 sequences of Chlamydomonas reinhardtii (CrMTD1), Gonium pectorale (GpMTD1), Yamagishiella unicocca (YuMTD1), Eudorina sp. (EuMTD1), Volvox reticuliferus (VrMTD1), Volvox africanus (VaMTD1) and Pleodorina starrii (PsMTD1, identified in this study) were aligned using the MUSCLE program built in the GENETYX-MAC software ver.

Article Title: HSF1 is required for cellular adaptation to daily temperature fluctuations
Article Snippet: The predicted amino acid sequences of HSFs from various vertebrate species were compared using GENETYX-MAC software (GENETYX Co., Tokyo, Japan).

Article Title: The CGA codon decoding through tRNA Arg (ICG) supply governed by Tad2/Tad3 in Saccharomyces cerevisiae.
Article Snippet: The detection by the anti-PGK1 antibody was used as a control. (B) Alignment of tR(ACG) gene sequences of S. cerevisiae from Saccharomyces genome database (SGD project, reference genome sequence R64-2-1) (tR(ACG)D, tR(ACG)E, tR(ACG)K, tR(ACG)L, tR(ACG)O and tR (ACG)J) aligned by GENETYX-MAC software (GENETYX Corporation, Tokyo, Japan).

Article Title: Real-time PCR assays to detect 10 Shiga toxin subtype (Stx1a, Stx1c, Stx1d, Stx2a, Stx2b, Stx2c, Stx2d, Stx2e, Stx2f, and Stx2g) genes.
Article Snippet: Todevelop subtypingmethods for Shiga toxin (Stx)1a, Stx1c, Stx1d, Stx2a, Stx2b, Stx2c, Stx2d, Stx2e, Stx2f, and Stx2g genes for epidemiological analyses of Shiga toxin-producing Escherichia coli (STEC), we developed 10 simplex realtimepolymerase chain reaction (PCR) assayswith reference to 284 valid stx sequences and evaluated their specificity and quantitative accuracy using STEC and non-STEC isolates and recombinant plasmids, respectively.. Three stx1 and 5 stx2 subtype genes, except for stx2c and stx2d, were detected with high specificity using STEC isolates.. However, some stx2a sequences potentially being close to both Stx2a and Stx2d cluster in neighbor-joining cluster analysis were positive for stx2a and stx2d by real-timePCR.

Article Title: Common host factors for internal ribosomal entry site-mediated translation of viral genomic RNA: An investigation in foot-and-mouth disease and classical swine fever viruses.
Article Snippet: DNA sequence characterization was performed using GENETYX-Mac software (GENETYX Co., Tokyo, Japan) and GENBANK.

Article Title: Use of universal primers for the 18S ribosomal RNA gene and whole soil DNAs to reveal the taxonomic structures of soil nematodes by high-throughput amplicon sequencing
Article Snippet: In brief, the nucleotide sequences were aligned, and phylogenetic trees were constructed using the BOOTSTRAP N-J TREE algorithm (bootstrap: 1000 replicates) with the ClustalX (version 2.1) package ( http://www.clustal.org/clustal2/ ) in the Genetyx-MAC software (version 19, Genetyx Co.) [ ].

Article Title: Evaluation of VP4-VP2 sequencing for molecular typing of human enteroviruses.
Article Snippet: Furthermore, pairwise identity comparison with gap was performed using the GENETYX-Mac software (version 20; GENETYX, Tokyo, Japan).

Article Title: Structural analyses of the GI.4 norovirus by cryo-electron microscopy and X-ray crystallography revealing binding sites for human monoclonal antibodies.
Article Snippet: Amino acid sequences of the P1 and P2 subdomains were aligned with the help of GENETYX-MAC software (Genetyx Corp., Tokyo).

Transfection:

Article Title: Reorganization of the ancestral sex-determining regions during the evolution of trioecy in Pleodorina starrii
Article Snippet: The MTD1 sequences of Chlamydomonas reinhardtii (CrMTD1), Gonium pectorale (GpMTD1), Yamagishiella unicocca (YuMTD1), Eudorina sp. (EuMTD1), Volvox reticuliferus (VrMTD1), Volvox africanus (VaMTD1) and Pleodorina starrii (PsMTD1, identified in this study) were aligned using the MUSCLE program built in the GENETYX-MAC software ver.

Article Title: HSF1 is required for cellular adaptation to daily temperature fluctuations
Article Snippet: The predicted amino acid sequences of HSFs from various vertebrate species were compared using GENETYX-MAC software (GENETYX Co., Tokyo, Japan).

Article Title: The CGA codon decoding through tRNA Arg (ICG) supply governed by Tad2/Tad3 in Saccharomyces cerevisiae.
Article Snippet: The detection by the anti-PGK1 antibody was used as a control. (B) Alignment of tR(ACG) gene sequences of S. cerevisiae from Saccharomyces genome database (SGD project, reference genome sequence R64-2-1) (tR(ACG)D, tR(ACG)E, tR(ACG)K, tR(ACG)L, tR(ACG)O and tR (ACG)J) aligned by GENETYX-MAC software (GENETYX Corporation, Tokyo, Japan).

Article Title: Real-time PCR assays to detect 10 Shiga toxin subtype (Stx1a, Stx1c, Stx1d, Stx2a, Stx2b, Stx2c, Stx2d, Stx2e, Stx2f, and Stx2g) genes.
Article Snippet: Todevelop subtypingmethods for Shiga toxin (Stx)1a, Stx1c, Stx1d, Stx2a, Stx2b, Stx2c, Stx2d, Stx2e, Stx2f, and Stx2g genes for epidemiological analyses of Shiga toxin-producing Escherichia coli (STEC), we developed 10 simplex realtimepolymerase chain reaction (PCR) assayswith reference to 284 valid stx sequences and evaluated their specificity and quantitative accuracy using STEC and non-STEC isolates and recombinant plasmids, respectively.. Three stx1 and 5 stx2 subtype genes, except for stx2c and stx2d, were detected with high specificity using STEC isolates.. However, some stx2a sequences potentially being close to both Stx2a and Stx2d cluster in neighbor-joining cluster analysis were positive for stx2a and stx2d by real-timePCR.

Article Title: Common host factors for internal ribosomal entry site-mediated translation of viral genomic RNA: An investigation in foot-and-mouth disease and classical swine fever viruses.
Article Snippet: DNA sequence characterization was performed using GENETYX-Mac software (GENETYX Co., Tokyo, Japan) and GENBANK.

Article Title: Use of universal primers for the 18S ribosomal RNA gene and whole soil DNAs to reveal the taxonomic structures of soil nematodes by high-throughput amplicon sequencing
Article Snippet: In brief, the nucleotide sequences were aligned, and phylogenetic trees were constructed using the BOOTSTRAP N-J TREE algorithm (bootstrap: 1000 replicates) with the ClustalX (version 2.1) package ( http://www.clustal.org/clustal2/ ) in the Genetyx-MAC software (version 19, Genetyx Co.) [ ].

Article Title: Evaluation of VP4-VP2 sequencing for molecular typing of human enteroviruses.
Article Snippet: Furthermore, pairwise identity comparison with gap was performed using the GENETYX-Mac software (version 20; GENETYX, Tokyo, Japan).

Article Title: Structural analyses of the GI.4 norovirus by cryo-electron microscopy and X-ray crystallography revealing binding sites for human monoclonal antibodies.
Article Snippet: Amino acid sequences of the P1 and P2 subdomains were aligned with the help of GENETYX-MAC software (Genetyx Corp., Tokyo).

Plasmid Preparation:

Article Title: Reorganization of the ancestral sex-determining regions during the evolution of trioecy in Pleodorina starrii
Article Snippet: The MTD1 sequences of Chlamydomonas reinhardtii (CrMTD1), Gonium pectorale (GpMTD1), Yamagishiella unicocca (YuMTD1), Eudorina sp. (EuMTD1), Volvox reticuliferus (VrMTD1), Volvox africanus (VaMTD1) and Pleodorina starrii (PsMTD1, identified in this study) were aligned using the MUSCLE program built in the GENETYX-MAC software ver.

Article Title: HSF1 is required for cellular adaptation to daily temperature fluctuations
Article Snippet: The predicted amino acid sequences of HSFs from various vertebrate species were compared using GENETYX-MAC software (GENETYX Co., Tokyo, Japan).

Article Title: The CGA codon decoding through tRNA Arg (ICG) supply governed by Tad2/Tad3 in Saccharomyces cerevisiae.
Article Snippet: The detection by the anti-PGK1 antibody was used as a control. (B) Alignment of tR(ACG) gene sequences of S. cerevisiae from Saccharomyces genome database (SGD project, reference genome sequence R64-2-1) (tR(ACG)D, tR(ACG)E, tR(ACG)K, tR(ACG)L, tR(ACG)O and tR (ACG)J) aligned by GENETYX-MAC software (GENETYX Corporation, Tokyo, Japan).

Article Title: Real-time PCR assays to detect 10 Shiga toxin subtype (Stx1a, Stx1c, Stx1d, Stx2a, Stx2b, Stx2c, Stx2d, Stx2e, Stx2f, and Stx2g) genes.
Article Snippet: Todevelop subtypingmethods for Shiga toxin (Stx)1a, Stx1c, Stx1d, Stx2a, Stx2b, Stx2c, Stx2d, Stx2e, Stx2f, and Stx2g genes for epidemiological analyses of Shiga toxin-producing Escherichia coli (STEC), we developed 10 simplex realtimepolymerase chain reaction (PCR) assayswith reference to 284 valid stx sequences and evaluated their specificity and quantitative accuracy using STEC and non-STEC isolates and recombinant plasmids, respectively.. Three stx1 and 5 stx2 subtype genes, except for stx2c and stx2d, were detected with high specificity using STEC isolates.. However, some stx2a sequences potentially being close to both Stx2a and Stx2d cluster in neighbor-joining cluster analysis were positive for stx2a and stx2d by real-timePCR.

Article Title: Common host factors for internal ribosomal entry site-mediated translation of viral genomic RNA: An investigation in foot-and-mouth disease and classical swine fever viruses.
Article Snippet: DNA sequence characterization was performed using GENETYX-Mac software (GENETYX Co., Tokyo, Japan) and GENBANK.

Article Title: Use of universal primers for the 18S ribosomal RNA gene and whole soil DNAs to reveal the taxonomic structures of soil nematodes by high-throughput amplicon sequencing
Article Snippet: In brief, the nucleotide sequences were aligned, and phylogenetic trees were constructed using the BOOTSTRAP N-J TREE algorithm (bootstrap: 1000 replicates) with the ClustalX (version 2.1) package ( http://www.clustal.org/clustal2/ ) in the Genetyx-MAC software (version 19, Genetyx Co.) [ ].

Article Title: Evaluation of VP4-VP2 sequencing for molecular typing of human enteroviruses.
Article Snippet: Furthermore, pairwise identity comparison with gap was performed using the GENETYX-Mac software (version 20; GENETYX, Tokyo, Japan).

Article Title: Structural analyses of the GI.4 norovirus by cryo-electron microscopy and X-ray crystallography revealing binding sites for human monoclonal antibodies.
Article Snippet: Amino acid sequences of the P1 and P2 subdomains were aligned with the help of GENETYX-MAC software (Genetyx Corp., Tokyo).

Microarray:

Article Title: Reorganization of the ancestral sex-determining regions during the evolution of trioecy in Pleodorina starrii
Article Snippet: The MTD1 sequences of Chlamydomonas reinhardtii (CrMTD1), Gonium pectorale (GpMTD1), Yamagishiella unicocca (YuMTD1), Eudorina sp. (EuMTD1), Volvox reticuliferus (VrMTD1), Volvox africanus (VaMTD1) and Pleodorina starrii (PsMTD1, identified in this study) were aligned using the MUSCLE program built in the GENETYX-MAC software ver.

Article Title: HSF1 is required for cellular adaptation to daily temperature fluctuations
Article Snippet: The predicted amino acid sequences of HSFs from various vertebrate species were compared using GENETYX-MAC software (GENETYX Co., Tokyo, Japan).

Article Title: The CGA codon decoding through tRNA Arg (ICG) supply governed by Tad2/Tad3 in Saccharomyces cerevisiae.
Article Snippet: The detection by the anti-PGK1 antibody was used as a control. (B) Alignment of tR(ACG) gene sequences of S. cerevisiae from Saccharomyces genome database (SGD project, reference genome sequence R64-2-1) (tR(ACG)D, tR(ACG)E, tR(ACG)K, tR(ACG)L, tR(ACG)O and tR (ACG)J) aligned by GENETYX-MAC software (GENETYX Corporation, Tokyo, Japan).

Article Title: Real-time PCR assays to detect 10 Shiga toxin subtype (Stx1a, Stx1c, Stx1d, Stx2a, Stx2b, Stx2c, Stx2d, Stx2e, Stx2f, and Stx2g) genes.
Article Snippet: Todevelop subtypingmethods for Shiga toxin (Stx)1a, Stx1c, Stx1d, Stx2a, Stx2b, Stx2c, Stx2d, Stx2e, Stx2f, and Stx2g genes for epidemiological analyses of Shiga toxin-producing Escherichia coli (STEC), we developed 10 simplex realtimepolymerase chain reaction (PCR) assayswith reference to 284 valid stx sequences and evaluated their specificity and quantitative accuracy using STEC and non-STEC isolates and recombinant plasmids, respectively.. Three stx1 and 5 stx2 subtype genes, except for stx2c and stx2d, were detected with high specificity using STEC isolates.. However, some stx2a sequences potentially being close to both Stx2a and Stx2d cluster in neighbor-joining cluster analysis were positive for stx2a and stx2d by real-timePCR.

Article Title: Common host factors for internal ribosomal entry site-mediated translation of viral genomic RNA: An investigation in foot-and-mouth disease and classical swine fever viruses.
Article Snippet: DNA sequence characterization was performed using GENETYX-Mac software (GENETYX Co., Tokyo, Japan) and GENBANK.

Article Title: Use of universal primers for the 18S ribosomal RNA gene and whole soil DNAs to reveal the taxonomic structures of soil nematodes by high-throughput amplicon sequencing
Article Snippet: In brief, the nucleotide sequences were aligned, and phylogenetic trees were constructed using the BOOTSTRAP N-J TREE algorithm (bootstrap: 1000 replicates) with the ClustalX (version 2.1) package ( http://www.clustal.org/clustal2/ ) in the Genetyx-MAC software (version 19, Genetyx Co.) [ ].

Article Title: Evaluation of VP4-VP2 sequencing for molecular typing of human enteroviruses.
Article Snippet: Furthermore, pairwise identity comparison with gap was performed using the GENETYX-Mac software (version 20; GENETYX, Tokyo, Japan).

Article Title: Structural analyses of the GI.4 norovirus by cryo-electron microscopy and X-ray crystallography revealing binding sites for human monoclonal antibodies.
Article Snippet: Amino acid sequences of the P1 and P2 subdomains were aligned with the help of GENETYX-MAC software (Genetyx Corp., Tokyo).

Isolation:

Article Title: Reorganization of the ancestral sex-determining regions during the evolution of trioecy in Pleodorina starrii
Article Snippet: The MTD1 sequences of Chlamydomonas reinhardtii (CrMTD1), Gonium pectorale (GpMTD1), Yamagishiella unicocca (YuMTD1), Eudorina sp. (EuMTD1), Volvox reticuliferus (VrMTD1), Volvox africanus (VaMTD1) and Pleodorina starrii (PsMTD1, identified in this study) were aligned using the MUSCLE program built in the GENETYX-MAC software ver.

Article Title: HSF1 is required for cellular adaptation to daily temperature fluctuations
Article Snippet: The predicted amino acid sequences of HSFs from various vertebrate species were compared using GENETYX-MAC software (GENETYX Co., Tokyo, Japan).

Article Title: The CGA codon decoding through tRNA Arg (ICG) supply governed by Tad2/Tad3 in Saccharomyces cerevisiae.
Article Snippet: The detection by the anti-PGK1 antibody was used as a control. (B) Alignment of tR(ACG) gene sequences of S. cerevisiae from Saccharomyces genome database (SGD project, reference genome sequence R64-2-1) (tR(ACG)D, tR(ACG)E, tR(ACG)K, tR(ACG)L, tR(ACG)O and tR (ACG)J) aligned by GENETYX-MAC software (GENETYX Corporation, Tokyo, Japan).

Article Title: Real-time PCR assays to detect 10 Shiga toxin subtype (Stx1a, Stx1c, Stx1d, Stx2a, Stx2b, Stx2c, Stx2d, Stx2e, Stx2f, and Stx2g) genes.
Article Snippet: Todevelop subtypingmethods for Shiga toxin (Stx)1a, Stx1c, Stx1d, Stx2a, Stx2b, Stx2c, Stx2d, Stx2e, Stx2f, and Stx2g genes for epidemiological analyses of Shiga toxin-producing Escherichia coli (STEC), we developed 10 simplex realtimepolymerase chain reaction (PCR) assayswith reference to 284 valid stx sequences and evaluated their specificity and quantitative accuracy using STEC and non-STEC isolates and recombinant plasmids, respectively.. Three stx1 and 5 stx2 subtype genes, except for stx2c and stx2d, were detected with high specificity using STEC isolates.. However, some stx2a sequences potentially being close to both Stx2a and Stx2d cluster in neighbor-joining cluster analysis were positive for stx2a and stx2d by real-timePCR.

Article Title: Common host factors for internal ribosomal entry site-mediated translation of viral genomic RNA: An investigation in foot-and-mouth disease and classical swine fever viruses.
Article Snippet: DNA sequence characterization was performed using GENETYX-Mac software (GENETYX Co., Tokyo, Japan) and GENBANK.

Article Title: Use of universal primers for the 18S ribosomal RNA gene and whole soil DNAs to reveal the taxonomic structures of soil nematodes by high-throughput amplicon sequencing
Article Snippet: In brief, the nucleotide sequences were aligned, and phylogenetic trees were constructed using the BOOTSTRAP N-J TREE algorithm (bootstrap: 1000 replicates) with the ClustalX (version 2.1) package ( http://www.clustal.org/clustal2/ ) in the Genetyx-MAC software (version 19, Genetyx Co.) [ ].

Article Title: Evaluation of VP4-VP2 sequencing for molecular typing of human enteroviruses.
Article Snippet: Furthermore, pairwise identity comparison with gap was performed using the GENETYX-Mac software (version 20; GENETYX, Tokyo, Japan).

Article Title: Structural analyses of the GI.4 norovirus by cryo-electron microscopy and X-ray crystallography revealing binding sites for human monoclonal antibodies.
Article Snippet: Amino acid sequences of the P1 and P2 subdomains were aligned with the help of GENETYX-MAC software (Genetyx Corp., Tokyo).

Biomarker Discovery:

Article Title: Reorganization of the ancestral sex-determining regions during the evolution of trioecy in Pleodorina starrii
Article Snippet: The MTD1 sequences of Chlamydomonas reinhardtii (CrMTD1), Gonium pectorale (GpMTD1), Yamagishiella unicocca (YuMTD1), Eudorina sp. (EuMTD1), Volvox reticuliferus (VrMTD1), Volvox africanus (VaMTD1) and Pleodorina starrii (PsMTD1, identified in this study) were aligned using the MUSCLE program built in the GENETYX-MAC software ver.

Article Title: HSF1 is required for cellular adaptation to daily temperature fluctuations
Article Snippet: The predicted amino acid sequences of HSFs from various vertebrate species were compared using GENETYX-MAC software (GENETYX Co., Tokyo, Japan).

Article Title: The CGA codon decoding through tRNA Arg (ICG) supply governed by Tad2/Tad3 in Saccharomyces cerevisiae.
Article Snippet: The detection by the anti-PGK1 antibody was used as a control. (B) Alignment of tR(ACG) gene sequences of S. cerevisiae from Saccharomyces genome database (SGD project, reference genome sequence R64-2-1) (tR(ACG)D, tR(ACG)E, tR(ACG)K, tR(ACG)L, tR(ACG)O and tR (ACG)J) aligned by GENETYX-MAC software (GENETYX Corporation, Tokyo, Japan).

Article Title: Real-time PCR assays to detect 10 Shiga toxin subtype (Stx1a, Stx1c, Stx1d, Stx2a, Stx2b, Stx2c, Stx2d, Stx2e, Stx2f, and Stx2g) genes.
Article Snippet: Todevelop subtypingmethods for Shiga toxin (Stx)1a, Stx1c, Stx1d, Stx2a, Stx2b, Stx2c, Stx2d, Stx2e, Stx2f, and Stx2g genes for epidemiological analyses of Shiga toxin-producing Escherichia coli (STEC), we developed 10 simplex realtimepolymerase chain reaction (PCR) assayswith reference to 284 valid stx sequences and evaluated their specificity and quantitative accuracy using STEC and non-STEC isolates and recombinant plasmids, respectively.. Three stx1 and 5 stx2 subtype genes, except for stx2c and stx2d, were detected with high specificity using STEC isolates.. However, some stx2a sequences potentially being close to both Stx2a and Stx2d cluster in neighbor-joining cluster analysis were positive for stx2a and stx2d by real-timePCR.

Article Title: Common host factors for internal ribosomal entry site-mediated translation of viral genomic RNA: An investigation in foot-and-mouth disease and classical swine fever viruses.
Article Snippet: DNA sequence characterization was performed using GENETYX-Mac software (GENETYX Co., Tokyo, Japan) and GENBANK.

Article Title: Use of universal primers for the 18S ribosomal RNA gene and whole soil DNAs to reveal the taxonomic structures of soil nematodes by high-throughput amplicon sequencing
Article Snippet: In brief, the nucleotide sequences were aligned, and phylogenetic trees were constructed using the BOOTSTRAP N-J TREE algorithm (bootstrap: 1000 replicates) with the ClustalX (version 2.1) package ( http://www.clustal.org/clustal2/ ) in the Genetyx-MAC software (version 19, Genetyx Co.) [ ].

Article Title: Evaluation of VP4-VP2 sequencing for molecular typing of human enteroviruses.
Article Snippet: Furthermore, pairwise identity comparison with gap was performed using the GENETYX-Mac software (version 20; GENETYX, Tokyo, Japan).

Article Title: Structural analyses of the GI.4 norovirus by cryo-electron microscopy and X-ray crystallography revealing binding sites for human monoclonal antibodies.
Article Snippet: Amino acid sequences of the P1 and P2 subdomains were aligned with the help of GENETYX-MAC software (Genetyx Corp., Tokyo).

Quantitative RT-PCR:

Article Title: Reorganization of the ancestral sex-determining regions during the evolution of trioecy in Pleodorina starrii
Article Snippet: The MTD1 sequences of Chlamydomonas reinhardtii (CrMTD1), Gonium pectorale (GpMTD1), Yamagishiella unicocca (YuMTD1), Eudorina sp. (EuMTD1), Volvox reticuliferus (VrMTD1), Volvox africanus (VaMTD1) and Pleodorina starrii (PsMTD1, identified in this study) were aligned using the MUSCLE program built in the GENETYX-MAC software ver.

Article Title: HSF1 is required for cellular adaptation to daily temperature fluctuations
Article Snippet: The predicted amino acid sequences of HSFs from various vertebrate species were compared using GENETYX-MAC software (GENETYX Co., Tokyo, Japan).

Article Title: The CGA codon decoding through tRNA Arg (ICG) supply governed by Tad2/Tad3 in Saccharomyces cerevisiae.
Article Snippet: The detection by the anti-PGK1 antibody was used as a control. (B) Alignment of tR(ACG) gene sequences of S. cerevisiae from Saccharomyces genome database (SGD project, reference genome sequence R64-2-1) (tR(ACG)D, tR(ACG)E, tR(ACG)K, tR(ACG)L, tR(ACG)O and tR (ACG)J) aligned by GENETYX-MAC software (GENETYX Corporation, Tokyo, Japan).

Article Title: Real-time PCR assays to detect 10 Shiga toxin subtype (Stx1a, Stx1c, Stx1d, Stx2a, Stx2b, Stx2c, Stx2d, Stx2e, Stx2f, and Stx2g) genes.
Article Snippet: Todevelop subtypingmethods for Shiga toxin (Stx)1a, Stx1c, Stx1d, Stx2a, Stx2b, Stx2c, Stx2d, Stx2e, Stx2f, and Stx2g genes for epidemiological analyses of Shiga toxin-producing Escherichia coli (STEC), we developed 10 simplex realtimepolymerase chain reaction (PCR) assayswith reference to 284 valid stx sequences and evaluated their specificity and quantitative accuracy using STEC and non-STEC isolates and recombinant plasmids, respectively.. Three stx1 and 5 stx2 subtype genes, except for stx2c and stx2d, were detected with high specificity using STEC isolates.. However, some stx2a sequences potentially being close to both Stx2a and Stx2d cluster in neighbor-joining cluster analysis were positive for stx2a and stx2d by real-timePCR.

Article Title: Common host factors for internal ribosomal entry site-mediated translation of viral genomic RNA: An investigation in foot-and-mouth disease and classical swine fever viruses.
Article Snippet: DNA sequence characterization was performed using GENETYX-Mac software (GENETYX Co., Tokyo, Japan) and GENBANK.

Article Title: Use of universal primers for the 18S ribosomal RNA gene and whole soil DNAs to reveal the taxonomic structures of soil nematodes by high-throughput amplicon sequencing
Article Snippet: In brief, the nucleotide sequences were aligned, and phylogenetic trees were constructed using the BOOTSTRAP N-J TREE algorithm (bootstrap: 1000 replicates) with the ClustalX (version 2.1) package ( http://www.clustal.org/clustal2/ ) in the Genetyx-MAC software (version 19, Genetyx Co.) [ ].

Article Title: Evaluation of VP4-VP2 sequencing for molecular typing of human enteroviruses.
Article Snippet: Furthermore, pairwise identity comparison with gap was performed using the GENETYX-Mac software (version 20; GENETYX, Tokyo, Japan).

Article Title: Structural analyses of the GI.4 norovirus by cryo-electron microscopy and X-ray crystallography revealing binding sites for human monoclonal antibodies.
Article Snippet: Amino acid sequences of the P1 and P2 subdomains were aligned with the help of GENETYX-MAC software (Genetyx Corp., Tokyo).

Over Expression:

Article Title: Reorganization of the ancestral sex-determining regions during the evolution of trioecy in Pleodorina starrii
Article Snippet: The MTD1 sequences of Chlamydomonas reinhardtii (CrMTD1), Gonium pectorale (GpMTD1), Yamagishiella unicocca (YuMTD1), Eudorina sp. (EuMTD1), Volvox reticuliferus (VrMTD1), Volvox africanus (VaMTD1) and Pleodorina starrii (PsMTD1, identified in this study) were aligned using the MUSCLE program built in the GENETYX-MAC software ver.

Article Title: HSF1 is required for cellular adaptation to daily temperature fluctuations
Article Snippet: The predicted amino acid sequences of HSFs from various vertebrate species were compared using GENETYX-MAC software (GENETYX Co., Tokyo, Japan).

Article Title: The CGA codon decoding through tRNA Arg (ICG) supply governed by Tad2/Tad3 in Saccharomyces cerevisiae.
Article Snippet: The detection by the anti-PGK1 antibody was used as a control. (B) Alignment of tR(ACG) gene sequences of S. cerevisiae from Saccharomyces genome database (SGD project, reference genome sequence R64-2-1) (tR(ACG)D, tR(ACG)E, tR(ACG)K, tR(ACG)L, tR(ACG)O and tR (ACG)J) aligned by GENETYX-MAC software (GENETYX Corporation, Tokyo, Japan).

Article Title: Real-time PCR assays to detect 10 Shiga toxin subtype (Stx1a, Stx1c, Stx1d, Stx2a, Stx2b, Stx2c, Stx2d, Stx2e, Stx2f, and Stx2g) genes.
Article Snippet: Todevelop subtypingmethods for Shiga toxin (Stx)1a, Stx1c, Stx1d, Stx2a, Stx2b, Stx2c, Stx2d, Stx2e, Stx2f, and Stx2g genes for epidemiological analyses of Shiga toxin-producing Escherichia coli (STEC), we developed 10 simplex realtimepolymerase chain reaction (PCR) assayswith reference to 284 valid stx sequences and evaluated their specificity and quantitative accuracy using STEC and non-STEC isolates and recombinant plasmids, respectively.. Three stx1 and 5 stx2 subtype genes, except for stx2c and stx2d, were detected with high specificity using STEC isolates.. However, some stx2a sequences potentially being close to both Stx2a and Stx2d cluster in neighbor-joining cluster analysis were positive for stx2a and stx2d by real-timePCR.

Article Title: Common host factors for internal ribosomal entry site-mediated translation of viral genomic RNA: An investigation in foot-and-mouth disease and classical swine fever viruses.
Article Snippet: DNA sequence characterization was performed using GENETYX-Mac software (GENETYX Co., Tokyo, Japan) and GENBANK.

Article Title: Use of universal primers for the 18S ribosomal RNA gene and whole soil DNAs to reveal the taxonomic structures of soil nematodes by high-throughput amplicon sequencing
Article Snippet: In brief, the nucleotide sequences were aligned, and phylogenetic trees were constructed using the BOOTSTRAP N-J TREE algorithm (bootstrap: 1000 replicates) with the ClustalX (version 2.1) package ( http://www.clustal.org/clustal2/ ) in the Genetyx-MAC software (version 19, Genetyx Co.) [ ].

Article Title: Evaluation of VP4-VP2 sequencing for molecular typing of human enteroviruses.
Article Snippet: Furthermore, pairwise identity comparison with gap was performed using the GENETYX-Mac software (version 20; GENETYX, Tokyo, Japan).

Article Title: Structural analyses of the GI.4 norovirus by cryo-electron microscopy and X-ray crystallography revealing binding sites for human monoclonal antibodies.
Article Snippet: Amino acid sequences of the P1 and P2 subdomains were aligned with the help of GENETYX-MAC software (Genetyx Corp., Tokyo).

Sequencing:

Article Title: Reorganization of the ancestral sex-determining regions during the evolution of trioecy in Pleodorina starrii
Article Snippet: The MTD1 sequences of Chlamydomonas reinhardtii (CrMTD1), Gonium pectorale (GpMTD1), Yamagishiella unicocca (YuMTD1), Eudorina sp. (EuMTD1), Volvox reticuliferus (VrMTD1), Volvox africanus (VaMTD1) and Pleodorina starrii (PsMTD1, identified in this study) were aligned using the MUSCLE program built in the GENETYX-MAC software ver.

Article Title: HSF1 is required for cellular adaptation to daily temperature fluctuations
Article Snippet: The predicted amino acid sequences of HSFs from various vertebrate species were compared using GENETYX-MAC software (GENETYX Co., Tokyo, Japan).

Article Title: The CGA codon decoding through tRNA Arg (ICG) supply governed by Tad2/Tad3 in Saccharomyces cerevisiae.
Article Snippet: The detection by the anti-PGK1 antibody was used as a control. (B) Alignment of tR(ACG) gene sequences of S. cerevisiae from Saccharomyces genome database (SGD project, reference genome sequence R64-2-1) (tR(ACG)D, tR(ACG)E, tR(ACG)K, tR(ACG)L, tR(ACG)O and tR (ACG)J) aligned by GENETYX-MAC software (GENETYX Corporation, Tokyo, Japan).

Article Title: Real-time PCR assays to detect 10 Shiga toxin subtype (Stx1a, Stx1c, Stx1d, Stx2a, Stx2b, Stx2c, Stx2d, Stx2e, Stx2f, and Stx2g) genes.
Article Snippet: Todevelop subtypingmethods for Shiga toxin (Stx)1a, Stx1c, Stx1d, Stx2a, Stx2b, Stx2c, Stx2d, Stx2e, Stx2f, and Stx2g genes for epidemiological analyses of Shiga toxin-producing Escherichia coli (STEC), we developed 10 simplex realtimepolymerase chain reaction (PCR) assayswith reference to 284 valid stx sequences and evaluated their specificity and quantitative accuracy using STEC and non-STEC isolates and recombinant plasmids, respectively.. Three stx1 and 5 stx2 subtype genes, except for stx2c and stx2d, were detected with high specificity using STEC isolates.. However, some stx2a sequences potentially being close to both Stx2a and Stx2d cluster in neighbor-joining cluster analysis were positive for stx2a and stx2d by real-timePCR.

Article Title: Common host factors for internal ribosomal entry site-mediated translation of viral genomic RNA: An investigation in foot-and-mouth disease and classical swine fever viruses.
Article Snippet: DNA sequence characterization was performed using GENETYX-Mac software (GENETYX Co., Tokyo, Japan) and GENBANK.

Article Title: Use of universal primers for the 18S ribosomal RNA gene and whole soil DNAs to reveal the taxonomic structures of soil nematodes by high-throughput amplicon sequencing
Article Snippet: In brief, the nucleotide sequences were aligned, and phylogenetic trees were constructed using the BOOTSTRAP N-J TREE algorithm (bootstrap: 1000 replicates) with the ClustalX (version 2.1) package ( http://www.clustal.org/clustal2/ ) in the Genetyx-MAC software (version 19, Genetyx Co.) [ ].

Article Title: Evaluation of VP4-VP2 sequencing for molecular typing of human enteroviruses.
Article Snippet: Furthermore, pairwise identity comparison with gap was performed using the GENETYX-Mac software (version 20; GENETYX, Tokyo, Japan).

Article Title: Structural analyses of the GI.4 norovirus by cryo-electron microscopy and X-ray crystallography revealing binding sites for human monoclonal antibodies.
Article Snippet: Amino acid sequences of the P1 and P2 subdomains were aligned with the help of GENETYX-MAC software (Genetyx Corp., Tokyo).



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